Childhood leukaemia and lymphoma: African experience supports a role for environmental factors in leukaemogenesis
Christopher KO Williams1, 2, 3, Letizia Foroni4, Lucio Luzzatto4, Idris Saliu1, Arthur Levine4, 5 and Mel F Greaves6
1Department of Haematology, College of Medicine, University of Ibadan, Ibadan, Nigeria
2Hematology Oncology Consultancy, Port Angeles, WA 98362, USA
3FHCRC/UW Centre for AIDS Research (CFAR), Seattle, WA 98104, USA
4Department of Haematology, Royal Postgraduate Medicine School, London W12 ONN, England
5Viral Epidemiology Branch, Landow Building 3C19, National Cancer Institute, Bethesda, MD 20205, USA
6Chester Beatty Laboratories, London SW3 6JB, England
Correspondence to: Christopher KO Williams. Email: firstname.lastname@example.org
Major differences exist in the nature of leukaemia and lymphoma in low-income African children compared to those in the high-income countries. These include the absence of the peak incidence of acute lymphoblastic leukaemia (ALL) in under-five-year olds that characterizes the disease in high-income countries. Conversely, chloroma association with acute myelogenous leukaemia (CA-AML/AMML) and Burkitt’s lymphoma (BL) are rare in the high-income countries. This report describes clinical and laboratory as well as epidemiological features of childhood leukaemia and lymphoma reported betwen 1982 and 1984 in the city of Ibadan, Nigeria. The observed pattern of distribution of childhood haematological malignancies in the city is more consistent with the observations of Ludwik Gross’s experiments on environmental influences, such as malnutrition and infections, animal leukaemogenesis, and mirroring the consequences of the primordial pressures that have shaped human genetics and pathophysiology.
Keywords: childhood leukaemia, lymphoblastic, environmental factors, chloroma, leukaemogenesis, socio-economic, lifestyle
Paediatric neoplasia, though relatively rare, is important for the understanding of oncogenesis . Studies of the African childhood lymphoma, otherwise known as Burkitt’s lymphoma, in the 1960s and 1970s, led to the appreciation of the role of environmental factors in oncogenesis [2, 3]. Furthermore, the unique chromosomal aberrations that are associated with this disease led to the understanding of the role of molecular events in cancer aetiology . Acute lymphoblastic leukaemia has served as a model for the development of curative cancer management [5, 6].
Though the incidence of childhood cancers is similar throughout the world, major differences exist in many parts of the world with respect to childhood haematological malignancies [7, 8]. For example, the features of childhood leukaemia and lymphoma in low-income African countries are different from those of children in the high-income countries. These differences include: the rarity of leukaemia below the age of 5 in the former, unlike the peaking of the incidence of the disease in the age group in the latter [9–12]; the high incidence of Burkitt’s lymphoma (BL) in the former, and its rarity in the latter population. Although the incidence of acute myeloid leukaemia in children varies little, there is considerable variability in its clinical manifestations in various parts of the world. The disease, for instance, is frequently associated with solid-tumour formation (chloromas) in the developing countries, leading to its being designated as ‘chloroma-associated acute myeloid leukaemia (CA-AML/AMML)’ [13, 14], with clinical features that make it almost indistinguishable from the more common BL.
Several factors have been implicated in the past in the aetiology of childhood ALL . In the study of the geographical distribution of leukaemia subtypes, Greaves and his colleagues  observed a striking difference in the proportion of the common acute lymphoblastic leukaemia (c-ALL) subtype between the affluent population of the United States, Europe, and South Africa and the less affluent populations of Nigeria and South Africa. Some of these observations led Greaves to postulate the hypothesis of ‘aberrant immune response’ to common infection(s) as a plausible aetiological mechanism for ALL .
The objective of this report is to seek explanation about the variations in the frequency of paediatric haematological cancers in the light of the varying lifestyles in communities.
Patients and methods
The patients included in this study were seen and assessed clinically at the University College Hospital (UCH), Ibadan, Nigeria. Initial laboratory diagnostic tests included a complete blood count (CBC) including differential leucocyte count on a Romanovsky-stained blood film. Bone marrow aspirates were routinely obtained in all cases of acute leukaemia and lymphoma and films prepared therewith were routinely processed with May Gruenwald Giemsa stain, and, in cases of acute leukaemia, with periodic acid Schiff (PAS) and Sudan Black stains. Haematoxylin Eosin-stained tissue sections were routinely obtained for the diagnosis of malignant lymphoma. Cells for immunophenotypic characterization were obtained from the tissues involved that could be conveniently sampled. Thus, heparinized peripheral blood was obtained in the cases of acute or chronic leukaemia with a total WBC in excess of 20.0×109/L, while in other cases heparinized bone marrow blood was utilized. In the cases of malignant lymphoma, the samples were obtained in the forms of cerebro spinal, ascitic or pleural fluids, biopsy of enlarged lymph nodes, or the involved viscera. When necessary, such samples were teased to release an adequate quantity of cells for the procedures.
The laboratory procedures of immunophenotypic characterization were performed according to the protocol of the International Study of Cell Markers in Leukaemias and Lymphomas as outlined by Greaves et al , using a panel of first generation reagents including: J 5  and AL2 , both anti CALLA; DA 2, an anti HLA DR , WT 1, an anti T  and OKT11a, an anti E rosette receptor ; and My906, an anti myeloid [22, 23] monoclonal antibody. A large number of heterologous anti sera, such as anti Ig, anti kappa, anti lambda, and anti Tdt (terminal deoxynucleotidyl transferase) were also included in the panel ; anti T subset murine monoclonal antibodies, including OKT3, OKT4, OKT6, and OKT8 [25, 26]. The binding of the monoclonal antibodies to target cells was determined by indirect immunofluorescence with fluoresceinated goat anti mouse IgG (in case of anti Tdt: rabbit anti mouse IgG) or by direct immunofluorescence in case of detection of cell-surface immunoglobulin, using a Leitz Ortholux II fluorescence microscope with incident illumination.
Criteria for subtype characterization
Acute lymphoblastic leukaemia
Only cases of acute leukaemia that did not react with the myeloid monoclonal My906 (CD33) were diagnosed as ALL. The subtypes of ALL were defined according to the previously published algorithm for the interpretation of immunophenotypic patterns observed in the International Study of Cell Markers in Leukaemias and Lymphomas , however, with some modifications as outlined by Borowitz . The subsets were defined as follows: common-ALL: CALLA , DR , T (WT1/E/T11)-, smIg-, Tdt ; null-ALL: CALLA-, DR , T (WT1/E/T11)-, smIg-, Tdt-; T-ALL: cALLA /- DR /- T (WT1/E/T11) , smIg-, Tdt ; B-ALL: CALLA-, DR , Tdt-.
Gene rearrangement studies
Samples of mononuclear cells from a few of the patients stored at −80 °C for several weeks were shipped to London, England, where they were studied for the evidence of gene rearrangement using a methodology that has previously been described .
For the purpose of studying the association of leukaemia subtypes with lifestyles, the city of Ibadan was sub-categorized into three zones depending on the lifestyle and social structure of the areas—Zone 1: the indigenous, old, and largely unplanned area, inhabited mainly by indigenes of the city, most of them are farmers, petty traders, and semi-skilled labourers. Environmental sanitation in this zone is very poor, and literacy is low. The average annual income is also very low (less than US$1,000 per year). Zone 2: non-indigenous high-density area inhabited by mixed population of business people, petty traders, professionals, skilled and unskilled labourers from various parts of the country, mainly from the neighbouring Yoruba-speaking States of the Federation of Nigeria. The level of education is generally higher than in the indigenous areas. The average annual income is intermediate between those of Zones 1 and 3. Zone 3: low/medium population density areas, consisting largely of parkland estates and predominantly inhabited by business people, academics, and professionals. The literacy rate is high; average annual income is the highest of all the three zones, and the lifestyle is generally comparable to that of suburban Western Europe or United States. The average annual income of the inhabitants of Zone 3 areas is above $10,000.
Each study subject was assigned to one of five socio-economic status (SES) groups depending on the level of education and occupation:
SES Group 1: Highly educated, senior public officers, business executives (estimated annual income: $10,000 or more)
SES Group 2: Post-secondary school educated; middle-level public officers (estimated annual income: $5,000–$9,000)
SES Group 3: Post-primary school educated, lower-level public officers or institutional staff and skilled handworkers (estimated annual income: $2,000–$3,500)
SES Group 4: Primary school educated and unskilled hand-workers (estimated annual income: $1,000–$1,500)
SES Group 5: Illiterate peasant farmers and petty traders (estimated annual income: less than $1,000)
The population sizes of the various SES groups of the residents of Ibadan city were projected from the most recently available census figures of 1963, assuming a uniform growth rate of between 2.5% and 5.0% for all five SES groups among children and adults. The distribution of the total estimated population into the various socio-economic groups was based on the studies of Odebiyi  and Onibokun et al . The reports of these studies suggested that individuals of low-, medium-, and high- socio-economic groups in the city constituted 75%, 12.5%, and 12.5%, respectively. There was no useful information in deriving the sizes of the population of the three categories of residential zones.
Furthermore, the calculation of children and adult population within the various socio-economic groups rested on the assumption that the World Bank  estimation of 47% and 53% for children (aged less than 15 years) and adults (aged 15 and above) applied uniformly for all socio-economic groups.
Spatial and temporal clustering of a particular disorder was determined to have taken place, respectively, if at least two cases of the disorder occurred in individuals resident within a distance of 2 km of one another and within a time span of 6 months.
Table 1. Patterns of rearrangement of immunoglobin (IgH), and T-cell receptor (TCR) beta- and gamma-genes, correlated with HTLV-I Western blot serology and DNA in leukaemia and lymphoma patients.
Eighty-four cases of malignant lymphoproliferative disorders and leukaemia were immunophenotyped at the Department of Haematology, University College Hospital (UCH), Ibadan, Nigeria between September 1982 and December 1984. They included 17 cases of childhood (age <15 years) ALL, 20 of adult (age ≥15 years) ALL, 6 cases of AML, 23 of CLL and 18 of non Hodgkin’s lymphoma. Specimens were available from 32 of the immunophenotyped individuals for gene rearrangement studies, which were subsequently carried out in June 1986. Of these, only 19, which were suitable for DNA extraction could be studied for gene rearrangements. The categorization of the disease subtype was based on immunophenotypic characteristics (see above) as well as gene rearrangement features (Table 1).
International comparative studies
A comparison of the pattern of ALL subtypes among Nigerians as observed in this study with the reported observations in USA (including Caucasian and African–American patients), UK, and Malaysia is provided in Table 2. C-ALL was least frequent (22.2%) among Nigerian children, next to the frequency of this condition among Malaysian and African American children. T-ALL was with 38.9% the most frequently encountered ALL subtype among Nigerian children. The frequency of c-ALL among adult Nigerian ALL patients (40%) was also lower than the observation among white adult Americans (50.7%) and adult UK patients (54.8%), but the differences were much less striking than the observations among children. Again, T-ALL was with 55% the most common subtype of ALL among adult Nigerian patients. This was much higher than the observation among adult white Americans (28.8%) and adult UK patients (11.9%).
Table 2. Pattern of acute lymphoblastic subtypes in Nigerians compared with those of the United States, UK, and Malaysia, including numbers of cases and frequency (%).
Table 3. Comparative incidencea of subtypes of acute lymphoblastic leukaemia in Nigerian, United Kingdom and US American children.
An estimate of the incidence rates of ALL and its subtypes in Nigerian, British and American children are outlined in Table 3. This is based on a previous comparative study of the pattern of ALL in the three countries, using Ibadan, Nigeria data , Birch et al 1980,  and Young et al 1975  as well as those of Greaves et al 1981 , Royston et al 1983  and Bowman et al . Although the incidence of ALL among Nigerian children was less than a third of those of the Caucasian children of UK and USA, and just over 60% of that of Black American children, yet the incidence of T-ALL was not remarkably different in the four groups of children, ranging between 0.31 for Nigerian, through 0.35 and 0.38, respectively, for UK and White US, to 0.40 for Black US children, thus, serving as an internal control and indicating that the reduced incidence of other subtypes was unlikely to be due to under diagnosis. The incidence of c-ALL in the presumed Caucasian UK and White American children, however, at 1.83 and 1.91 respectively, was at least ten fold, and that of American Black children, estimated at 0.70, almost three times higher than that of Nigerian children, which has been estimated at 0.18. The incidence of B-ALL among Nigerian children, estimated at 0.18 is three to nine times higher than that of among Caucasian American and UK children with rates of 0.06 and 0.02, respectively. Thus, the main differences in the incidence of ALL in the four populations is principally attributable to the differences in the incidence of non T-ALL.
Time-space clustering of haematological malignancies
Thirty-five occurrences of time-space clustering of subtypes of haematological malignancies were observed. Twenty-eight (80%) of these were observed within Zone 1, 6 (17.1%) in Zone 2, and 1 (2.3%) in Zone 3. The highest frequency of clustering was observed with Burkitt’s lymphoma, among which 13 (93.9%) of 14 observations of clustering (involving 31 patients) occurred within Zone 1. There were four observations of AML/AMML clustering involving nine patients all in Zone 1, and 3 of ALL involving 8 patients, 2 in Zone 2 and 1 Zone 3 and none in Zone 1.
The incidence of Burkitt’s lymphoma at 1.81–3.62 was highest in Zone 1, compared to 0.21–0.42 in Zone 2 and 0 in Zone 3. The incidence of AML/AMML of was 0.41–0.42 in Zone 1, 0.18–0.37 in Zone 2 and Zone 3, while the incidence of ALL was with 0.75–1.51 highest in Zone 3 and lowest in Zone 1 at 0.25–0.50.
Figure 1 illustrates the relationship between hepatitis A seroprevalence, a marker of environmental sanitation and the incidence/mortality of childhood ALL in global communities, including Ibadan, Nigeria in the mid 1980s (this study), post-World War II Okinawa (before 1960 and after 1960), the United States (Caucasians, 1976/1980), and UK (1980 and 1988/89) . The apparent inverse relationship is consistent with the effect of environmental sanitation on childhood leukaemia incidence and mortality in the population.
Figure 1. Incidence of childhood leukaemia correlated with HAV seroprevalence in the first decade of life. US Cau. = US Caucasians. Data based on Smith et al  a. Acute lymphoblastic leukaemia in 2–4 year olds. b. HAV seroprevalence rates. In developing countries of Africa, Asia, Latin America, and pre-1960 Okinawa, Japan, where age-specific variations are not observed, cited infection rates represent cross-sectional values. In the developed countries of USA and United Kingdom as well as post-1960 Okinawa, Japan, the HAV values represent infection rates in the childbearing age of 25–30 years .
Key to Figure 1:
1. Solid rectangle: Leukaemia incidence in Ibadan, Nigeria as presented in this paper; HAV seroprevalence rates in Ibadan, Nigeria 1982 .
2. Upper closed diamond: US Caucasians 1921–1925 . Infant mortality rate , another measure of the adequacy of public hygiene , has been used as a surrogate index of HAV seroprevalence rate.
3. Lower closed diamond: US Caucasians 1956–1960 .
4. Open diamond: US Caucasians 1976-1980 (cross-sectional HAV rate used) .
5. Closed diamond: US Non Caucasians 1946–1950 .
6. Open rectangle: Okinawa, Japan, before 1960 .
7. Open rectangle with a dot: Okinawa, Japan 1980–1984.
8. Standing triangle: Bangalore, India .
9. Inverted triangle: Philippines, Manila 1988–1992 .
10. X-open circle: Peru, Lima, 1990–1991 .
One of the consequences of the development in recent times of monoclonal antibodies is that lymphoid neoplasias are now recognizable as heterogeneous with respect to the differentiation-associated markers present on their cells . A comparison of the patterns of ALL subtypes among Nigerian children and adults as observed in this study with the observations in British, American, and Malaysian studies (Table 2) reveals a marked deficit of c-ALL in the Nigerian children. If it is correct to assume that the Malaysians and Black Americans are socio-economically intermediate between Nigerians and the Caucasian populations of the UK and United States, one could suggest an apparent correlation between the relative frequency of c-ALL in the compared populations and their socio-economic status. It also appears reasonable to suggest that the most striking differences in the epidemiology of childhood ALL in Nigeria and the socio-economically developed countries is probably due to the deficit of c-ALL and excess of B-ALL (Table 3) among Nigerians. Ramot and Magrath  have highlighted the possible role of economic deprivation in the aetiology of Burkitt’s lymphoma, a condition that is believed to be related to B-ALL. Low socioeconomic status also characterized the population within which BL was prevalent in Eastern Nigeria . Indeed, any theory explaining the mechanism of deficit of c-ALL among African children should also be able to explain the concomitant excess occurrence of B-cell leukaemia and Burkitt’s lymphoma in the same population. Overall, the data presented in this communication are consistent with the fact that the lifestyle of socioeconomic deprivation with its hallmarks of malnutrition, sanitary deficiencies, and endemic infections, is associated with reduced incidence of c-ALL and excess of B-ALL subtypes, while the reverse is the case in high-income societies [9, 43].
In addition to the reduced incidence of c-ALL, and increased incidence of BL, the increased frequency of chloroma-associated acute myelogenous leukaemia (otherwise known as chloroleukaemia) is the another feature of childhood haematological malignancies in developing countries. With 5 of 8 cases (62.5%) of AML/AMML in under-5-year olds occurring in association with chloromas as compared to 2 of 18 (11%) in older individuals , chloroma-associated AML/AMML appears to be a specific disorder of childhood in Nigeria rather than a manifestation of late presentation. This form of AML/AMML presentation has been reported in various parts of the world, including Turkey , Egypt , and Central Africa . In a report from South Africa, 24% of black children but no Caucasian child presented with CA-AML . Chloroma-associated AML was reported in 3 of 162 cases in a United Kingdom report  and in 1 of 114 Caucasian children in an US report  thus making this form of AML presentation 28 times and 60 times more frequent in Nigerian children than in UK and in Caucasian US children, respectively . This observation is most probably related to biological variations resulting from differences in contemporary lifestyles, rather than ethnicity. Thus, in Ibadan, clustering of chloroma-associated AML/AMML was observed at 2-fold increase of incidence in areas inhabited by the people of low SES lifestyle, while ALL clustered at 3-fold increased incidence in areas inhabited by medium to high SES , thus suggesting a role for lifestyle associated environmental influences. Such influences appear to include the effects of social deprivation, infection, and the state of hygiene [40, 42] as well as malnutrition-related thymolymphatic and acquired immunodeficiency states [50, 51]. The more recent observation from another part of Nigeria  on the epidemiology of BL is consistent with this observation. Lack of reporting of chloroma cases in the recent literature on paediatric malignancies from Nigeria [8, 12] would tend to suggest that the condition is either not being recognized or it may be disappearing in the area. If the latter is the case, it may mirror the course of events in Great Britain, where chloromas were believed to have been more common in the past than in more recent years , thus leading to the speculation that chloroma frequency will diminish in African childhood pathology with the improvement of child health in the region .
The epidemiological features of childhood leukaemia in the low-income parts of Africa, with the reduced incidence of ALL and the increased incidence of chloroma-associated AML, are reminiscent of the experimental observations of Ludwik Gross on the influence of environmental factors in animal leukaemogenesis. Following the underfeeding of Ak mice, Gross observed a delay in the onset of and a reduction in the rate of occurrence of virus-induced leukaemia. Thymectomy also inhibited or considerably delayed the development of lymphatic leukaemia, and frequently caused the myelogenous forms to appear later in life, often in the form of chloroleukaemia . These observations have led to the hypothesis that childhood leukaemia/lymphoma epidemiology in Nigeria could be Nature’s equivalent of Ludwig Gross’s experiment, with thymo-lymphatic deficiency resulting from the combination of maternal and intra-uterine fetal malnutrition . The impact of SES on early life non-neoplastic diseases has been well documented by Aizer and Currie .
Biological and immunological derangement also appear to be the underlying rationale for the hypothesis of ‘delayed-infection’ advanced by Greaves to explain the pathogenesis of ALL in the 2–5 year age-group in ‘modern or affluent societies’ , and the ‘population mixing’ hypothesis of Leo Kinle .
For a biological model to be plausible in explaining the epidemiologic patterns of leukaemia/lymphoma subtypes in a developing society, such as Nigeria, it should explain the three main features of childhood leukaemia and lymphoma in Nigerians, namely, reduced incidence of c-ALL subtypes, increased incidence of Burkitt’s lymphoma, and the increased incidence of chloroma-associated AML/AMML. None of the three biological models discussed earlier satisfactorily explains the epidemiological patterns of childhood leukaemia/lymphoma described in this report. The two-fold increased incidence and clustering of chloroma-associated AML/AMML in the absence of clustering of, but two-fold reduced incidence of ALL in the low SES area of Ibadan appears to be most consistent with the studies of Ludwik Gross , except that the model did not specify the subtypes of leukaemia/lymphoma (which had not been discovered at the time of the experiments). The hypothesis of ‘delayed infection’  is consistent with the marked reduction of c-ALL subtype observed in this report, especially given the ubiquitous and prevalent relatively unhygienic status in a developing society, and hence ‘hyper-immune’ status as compared to the affluent society for which it has been construed . However, this hypothesis does not explain the high incidence of B-cell lymphoma subtypes and chloroma-associated AML/AMML as described in this report. The ‘population mixing’ hypothesis of Kinle  is consistent with the observation of clustering of ALL in the medium and high SES areas of Ibadan, given the free intermingling in these areas of children of varied life-styles, presumably including ‘immune’ and ‘non-immune’ ones. However, this hypothesis does not explain the frequent occurrence of choroma-associated AML/AMML in Nigerian children.
The global and temporal trend in the incidence and/or mortality of ALL in various parts of the world (Figure 1) appears to correlate with the state of sanitation and hygiene within communities, thus indicating a role for sanitation-related factors globally . It is unclear how or at what stage of life these environmental factors impact the human development. However, the observations correlate with observation of others as to the influence of maternal socioeconomic disadvantages and the health at birth. It is known that the fetus is particularly vulnerable to myriad health insults, some of which are preventable with appropriate health policies, including vaccination of pregnant women against influenza, supplementing food and nutrition of pregnant women through government programmes, and reduction of exposure to environmental toxins during pregnancy .
The observations described in this report date back a few decades. They are now being re-analysed and discussed in the light of newer development in our understanding of environmental influence on the disease processes of fetuses and children , including leukaemogenesis . Furthermore, and in spite of a number of limiting factors, including limited reliable population figures for Ibadan, paucity of the number of cases, and the quality of the laboratory facilities and reagents available at the time of the study, it is important to observe that, in the intervening period of time, no other study from the region has addressed the issues covered in this report [8, 11, 12].
Table 4. Estimated population sizes and incidencea of haemopoietic malignancies among three socio-economic groups of inhabitants of Ibadan, Nigeria (1979–1978).
The subject of the aetiology of childhood leukaemia and lymphoma remains relevant today in all parts of the world. The lifestyles of developing societies that appear to be the underlying causes of the unique features of childhood leukaemia and lymphoma described in this report (Table 4) persist, and appear to mirror the consequences of the primordial pressures that have shaped human genetics and pathophysiology , apparently mediated by the macrophages, which have been described as an evolutionary ancient cell type . Meanwhile, technological development, including flow cytometry for more accurate immunophenotypic cell characterization, availability of better reagents, and molecular biological methodologies for viral studies, among others, give us a unique opportunity to elucidate the mechanism of the interaction of genetic and environmental factors in childhood with the potential for new knowledge in leukaemia prevention and management.
The differences in the incidence and clinical manifestations of childhood leukaemias/lymphomas between the low-income regions of Africa and the high-income regions of the world are related to the differences in lifestyles that are prevalent in these varying parts of the world. They indicate the greater role of environmental pressures on leukaemogenesis compared to that of genetic differences of ethnicity and race. It is expected that as socio-economic differences among populations even out, so also will the observed differences in the epidemiological features of childhood leukaemia/lymphoma.
1. Knudson Jr AG (1993) Pediatric molecular oncology. Past as prologue to the future Cancer 71(S10) 3320–4 DOI: 10.1002/1097-0142(19930515)71:10 <3320::AID-CNCR2820711730>3.0.CO;2-1 PMID: 8490875
3. Geser A et al (1978) Epidemiological evidence for causal relationship between Epstein-Barr virus and Burkitt’s lymphoma from Ugandan prospective study Nature 274(5673) 756–61 DOI: 10.1038/274756a0 PMID: 210392
7. Magrath IT et al (1997) Pediatric Oncology in Countries With Limited Resources 3rd edn (Philadelphia and New York: Lippincott-Raven)
9. Williams CKO et al (2002) Proc. of 38th Annual Meeting of the American Society of Clinical Oncology on Reduced Incidence of Common Acute Lymphoblastic Leukaemia And Its Absence in The First Quinquenium in An African Population Is Consistent With a Role for Delayed Infection in Its Aetiology
11. Kagu M et al (2013) Spectrum of haematologic malignancies and survival outcomes of adult lymphomas in Maiduguri, north eastern Nigeria—a fourteen year review Afr J Med Med Sci 42(1) 5–14
17. Heimann R et al (1997) Race and clinical outcome in breast cancer in a series with long-term follow-up evaluation J Clin Oncol 15(6) 2329–37
18. Lebacq-Verheyden AM et al (1983) Rat AL2, AL3, AL4 and AL5 monoclonal antibodies bind to the common acute lymphoblastic leukaemia antigen (CALLA gp 100) Int J Cancer 32(3) 273–9 DOI: 10.1002/ijc.2910320303 PMID: 6576989
20. Tax W et al (1982) Monoclonal Antibody Against Human Thymocytes and T Lymphocytes., in Protides of the Biological Fluids, ed H Peeters (Pergamon Press: Oxford) pp 701–4
22. Greaves MF (1984) Subtypes of Acute Lymphoblastic Leukaemia: Implications for the Pathogenesis and Epidemiology of Leukaemia. Pathogenesis of Leukemia and Lymphoma Environmental Influences, eds I Magrath, GT O’Connor, and B Ramot (New York: Raven Press) p 129
23. Griffin JB and Schlossman SF (1984) Proc of the First International Conference on Leucocyte Differentiation Antigens, Expression of myeloid differentiation antigens in acute myeloblastic leukemia, eds E Milstein and SF Schlossman Springer-Verlag
26. Reinherz EL et al (1980) Proc National Academy of Sciences of the United States of America on Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage. 77(3) 1588–92 PMID: 6966400 PMCID: 348542
28. Stathopoulos G and Elliott E (1974) Formation of mouse or sheep redblood-cell rosettes by lymphocytes from normal and leukaemic individuals The Lancet 303(7858) 600–1 DOI: 10.1016/S0140-6736(74)92655-5
29. Greaves MF, Pegram SM, and Chan L (1985) Collaborative group study of the epidemiology of acute lymphoblastic leukaemia subtypes: background and first report Leuk Res 9(6) 715–33 DOI: 10.1016/0145-2126(85)90281-4 PMID: 3859718
31. Foroni L et al (1987) Alpha, beta and gamma T-cell receptor genes: rearrangements correlate with haematological phenotype in T cell leukaemias Brit J Haematol 67(3) 307–18 DOI: 10.1111/j.1365-2141.1987.tb02352.x PMID: 2961364
32. Odebiyi A (1980) Socio-economic status, illness, behaviour and attitudes towards disease etiology in Ibadan Niger Behav Sci J 3 172–86
33. Onibokun P, Adesokan R, and Bello I (eds) (1981) Oyo State: A Survey of Resources for Development, U.o.I. Nigerian Institute of Social and Economic Research, Ibadan, Nigeria, Editor. Ibadan, Nigeria
34. Anonymous, World Bank Report: Nigeria: Country Economic Memorandum. 1981, World Bank: Washington, DC, USA
35. Birch JM, Marsden HB, and Swindell R (1980) Incidence of malignant disease in childhood: a 24-year review of the Manchester Children’s Tumour Registry data Br J Cancer 42(2) 215–23 DOI: 10.1038/bjc.1980.221 PMID: 6252925 PMCID: 2010396
37. Greaves MF et al (1981) Immunologically defined subclasses of acute lymphoblastic leukaemia in children: their relationship to presentation features and prognosis Br J Haematol 48(2) 179–197 DOI: 10.1111/j.1365-2141.1981.tb02704.x
38. Royston I et al (1983) Phenotypes of adult acute lymphoblastic leukemia defined by monoclonal antibodies. Human leucocyte markers detected by monoclonal antibodies, ed. B.e. al
39. Bowman E et al (1984) A comparative analysis of acute lymphocytic leukemia in White and Black children: presenting clinical features and immunologic markers. Pathogenesis of leukemias and lymphomas: Environmental influences, eds I Magrath, G O’conor, and B Ramot (New York: Raven Press)
40. Smith MA et al (1998) Evidence that childhood acute lymphoblastic leukemia is associated with an infectious agent linked to hygiene conditions Cancer Causes Control 9(3) 285–98 DOI: 10.1023/A:1008873103921 PMID: 9684709
41. Greaves M et al (1981) Exploitation of monoclonal antibodies: a “who’s who” of haemopoietic malignancy Blood Cells 7(2) 257–80
42. Ramot B and Magrath I (1982) Hypothesis: the environment is a major determinant of the immunological sub-type of lymphoma and acute lymphoblastic leukaemia in children Br J Haematol 50(2) 183–9 DOI: 10.1111/j.1365-2141.1982.tb01908.x PMID: 6977370
43. Williams CKO (2012) Proc 103rd Annual Meeting of the American Association for Cancer Research on Childhood Leukemia and Lymphoma: African Experience Supports a Role for Environmental Factors
44. Williams C (1971) Neoplastic diseases of the haemopoietic system in Ibadan: preliminary report of a prospective study Afr J Med Sci 14 89–94
48. Macdougall LG et al (1986) Acute childhood leukemia in Johannesburg: Ethnic differences in incidence, cell type, and survival J Pediatr Hematol Oncol 8(1) 43–51 DOI: 10.1097/00043426-198608010-00009
49. Williams CKO (1988) Clustering of Burkitt’s lymphoma and other high-grade malignant lymphoproliferative diseases, but not acute lymphoblastic leukemia among socio-economically deprived Nigerians East Afr Med J 65(4) 253–63 PMID: 3168858
51. McFarlane H et al (1977) Evidence of impaired immunological response in malnourished human population. in XIII Symposium of the Swedish Nutrition Foundation
52. Hayhoe FGJ (1960) Leukaemia Little, Brown, Boston
54. Gross L (1970) Oncogenic Viruses. 2nd ed (Oxford: Pergamon Press)
55. Williams CKO (2013) 104th Annual Meeting of the American Association for Cancer Research on Epidemiology of Childhood Leukemia/Lymphoma in Resource-Poor Countries: Nature’s Manifestation of Ludwig Gross’s Experiments on Environmental Influence on Animal Leukemogenesis?
57. Kinlen L (1988) Evidence for an infective cause of childhood leukaemia: comparison of a Scottish new town with nuclear reprocessing sites in Britain The Lancet 332(8624) 1323–7 DOI: 10.1016/S0140-6736(88)90867-7 5247030
59. Greaves MF and Greaves M (2000) Cancer: The Evolutionary Legacy (Oxford University Press Oxford)
61. Lister T et al (1979) Prognostic significance of cell surface phenotype in adult acute lymphoblastic leukaemia Cancer Immunol Immunother 6(4) 227–30
63. Williams CK, Essien EM and Bamgboye EA (1984) Trends in Leukemia Incidence in Ibadan, Nigeria. Pathogenesis of Leukemia and Lymphoma: Environmental Influences, eds I Magrath, GT O’Connor, and B Ramot (New York: Raven Press) pp.17–27
65. Smith MA et al (1998) Evidence that childhood acute lymphoblastic leukemia is associated with an infectious agent linked to hygiene conditions Cancer Causes Control 9(3) 285–98
66. Ayoola E (1982) Antibody to hepatitis A virus in healthy Nigerians J Nat Med Assoc 74(5) 465
67. Anonymous, US Bureau Of Census: Historical Statistics of the United States, 1789–1945, U.B.O. Census, Editor. 1949, US Government Printing Office, 1949: Washington, DC
68. Bernal W, Smith H and Williams R (1996) A community prevalence study of antibodies to hepatitis A and E in inner-city London J Med Virol 49(3) 230–34