Human papillomavirus detection in head and neck squamous cell carcinoma

23 Oct 2014
Dayahindara Vietía, Juan Liuzzi, Maira Ávila, Zoraya De Guglielmo, Yrneh Prado, Maria Correnti

Introduction: Human Papillomavirus (HPV) has been associated with benign and malignant lesions in different epitheliums. The relationship between specific genotypes of high-risk HPV and some human cancers is well established. The aim of this work was to detect the HPV genotypes present in head and neck squamous cell carcinoma (HNSCC).

Methods: We evaluated 71 samples of patients with histopathological diagnosis of HNSCC. The DNA extraction was conducted with the QIAGEN commercial kit. HPV detection and genotyping were performed by reverse hybridisation (INNO-LiPA) following the commercial specifications.

Results: The mean age of the patients evaluated was 60.7 ± 13.11 years. The distribution of the lesions included 25 (35.20%) cases of squamous cell carcinoma (SCC) of the oral cavity, 23 (32.39%) of larynx, 16 (22.50%) of the oropharynx, 4 (5.63%) of paranasal sinus, and 2 (2. 80%) cases of SCC of the nostril. Of the patients, 78.9% were males, and of these 76% were tobacco users and 67.6% were alcohol consumers. The viral DNA was detected in 67.6% of the samples. The oral cavity and the larynx were the highest HPV-positivity sites with 35.40% and 29.10% respectively. The most frequent genotype was 16 as single infection (18.70%), or in combination with another HPV types. In the oral cavity and larynx were present the genotypes 16 or the combination 6 and 51 in 11.76% and 14.28% respectively; and in the oropharynx the most frequent genotype was 16 in 22.50% of the cases, and in the paranasal sinus 50% presented infection with HPV-6. We observed that tumours with most advanced size and stage presented greater HPV positivity.

Conclusions: This study shows a high percentage of HPV positivity in SCC is mainly associated with high-risk HPV. It is important to highlight that viral infection, especially HPV-16, could be a risk factor in HNSCC progression.

Related Articles

Goutam Santosh Panda, Vanita Noronha, Subhash Yadav, Amit Joshi, Vijay Patil, Nandini Menon, Rajiv Kumar, Amit Janu, Abhishek Mahajan, Nilendu Purandare, Jai Prakash Agarwal, George Karimundackal, Kumar Prabhash
Fitalew Tadele Admasu, Biruk Demissie, Getachew Yideg Yitbarek, Zeleke Geto, Aragaw Tesfaw, Edget Abebe Zewde, Animut Tilahun, Gashaw Walle, Tigist Tefera Bekele, Mezgebu Legesse Habte, Teka Obsa Feyisa, Tadeg Jemere Amare, Wubet Alebachew, Sintayehu Asnakew, Ermiyas Sisay, Markeshaw Tiruneh, Getaneh Atikilt Yemata, Tigabu Munye Aytenew, Tadesse Asmamaw Dejenie
Goutam Santosh Panda, Vanita Noronha, Darshit Shah, George John, Anuradha Chougule, Vijay Patil, Rajiv Kumar, Nandini Menon, Ajay Singh, Pratik Chandrani, Abhishek Mahajan, Kumar Prabhash
Arun Sankar Sudha, Ravikumar Rejnish Kumar, Milan Anjanappa, Cessal Thomas Kainickal, Aleyamma Mathew, Ramadas Kunnambath
Hasan Nassereldine, Hussein Awada, Adel Hajj Ali, Mohammad Zeineddine, Zahy Abdul Sater, Yasser Shaib