There is a growing demand for diagnostic markers for early disease detection and prognosis.

Exosomes are potential biomarkers for cancer progression and neurodegenerative disease but it can be difficult to identify what tissue a specific exosome comes from.

Researchers at Uppsala University have solved this problem by developing a method that maps surface protein complements on large numbers of individual exosomes.

Their work was published in Nature Communications.

Exosomes are released from all cells in the body.

They convey protein and nucleic acid cargos between the cells as a form of intercellular communication, and they represent potential circulating biomarkers for tumour progression and metastasis, as well as for early detection of neurodegenerative disease.

In order to use exosomes as biomarkers of diseases in different tissues it is vital to distinguish them according to their surface protein complements.

Researchers at Uppsala University, along with collaborators, have developed a method that can map surface protein complements on large numbers of individual exosomes.

The novel proximity-dependent barcoding assay (PBA) reveals the surface protein composition of individual exosomes using antibody-DNA conjugates and next-generation sequencing.

The method identifies proteins on individual exosomes using micrometer-sized, uniquely tagged single-stranded DNA clusters generated by rolling circle amplification.

"This new technology will allow large-scale screens for biomarkers in disease, complementing a panel of methods for sensitive and specific detection of exosomes that we have previously established," says Masood Kamali-Moghaddam one of the group leaders at the Molecular Tools unit at Uppsala University.

Source: Uppsala University