ecancermedicalscience

Research

High-risk gastrointestinal stromal tumour (GIST) and synovial sarcoma display similar angiogenic profiles: a nude mice xenograft study

9 Mar 2017
Francisco Giner, Isidro Machado, Jose Antonio Lopez-Guerrero, Empar Mayordomo-Aranda, Antonio Llombart-Bosch

Background: Gastrointestinal stromal tumour (GIST) is the most common primary mesenchymal tumour of the gastrointestinal tract. Spindle cell monophasic synovial sarcoma (SS) can be morphologically similar. Angiogenesis is a major factor for tumour growth and metastasis. Our aim was to compare the angiogenic expression profiles of high-risk GIST and spindle cell monophasic SS by histological, immunohistochemical and molecular characterisation of the neovascularisation established between xenotransplanted tumours and the host during the initial phases of growth in nude mice.

Methods: The angiogenic profile of two xenotransplanted human soft-tissue tumours were evaluated in 15 passages in nude mice using tissue microarrays (TMA). Tumour pieces were also implanted subcutaneously on the backs of 14 athymic Balb-c nude mice. The animals were sacrificed at 24, 48, and 96 h; and 7, 14, 21, and 28 days after implantation to perform histological, immunohistochemical, and molecular studies (neovascularisation experiments).

Results: Morphological similarities were apparent in the early stages of neoplastic growth of these two soft-tissue tumours throughout the passages in nude mice and in the two neovascularisation experiments. Immunohistochemistry demonstrated overexpression of proangiogenic factors between 24 h and 96 h after xenotransplantation in both tumours. Additionally, neoplastic cells coexpressed chemokines (CXCL9, CXCL10, GRO, and CXCL12) and their receptors in both tumours. Molecular studies showed two expression profiles, revealing an early and a late phase in the angiogenic process.

Conclusion: This model could provide information on the early stages of the angiogenic process in monophasic spindle cell SS and high-risk GIST and offers an excellent way to study possible tumour response to antiangiogenic drugs.

Article metrics: 731 views
310
421

Related Articles

Gustavo Duarte Ramos Matos, Veridiana Pires de Camargo, Rodrigo Ramella Munhoz, Gilberto de Castro Jr
Ethan A Burns, Amna Ahmed, Anusha Sunkara, Usman Khan, Roozbeh Sharif, Maen Abdelrahim, Michael Reardon, Barry Trachtenberg
Chiara De Angelis, Paolo Della Vigna, Gianluca Maria Varano, Giovanni Mauri
Rafael Delgado Morales, Armando Gil Mendoza, Carmen Luces, Afren Bolívar Abreu, Gabriel Romero, Gabriel Pérez, Leonardo Russo