Short Communication

Detection of methylation in the CpG islands of the P16INK4A, RASSF 1A and methylguanine methyltransferase (MGMT) gene promoters in pancreatic adenocarcinoma

15 Jan 2009
D Paikos, P Stravoravdi, S Voyatzi, I Boukovinas, P Papakotoulas, A Kiziridou, G Sibilidis, I Stergiou

Pancreatic cancer consists of an accumulation of genetic and epigenetic alterations. Recently, aberrant methylation of CpG islands of cancer-related genes has emerged as an important epigenetic mechanism of their transcriptional dysregulation during tumour development [1]. Therefore, new diagnostic methods, for early detection based on a better understanding of the molecular biology of pancreatic cancer, are required. We examined the methylation status of p16INK4A, RASSF 1A and methylguanine methyltransferase (MGMT) genes considered to be inactivated by promoter methylation in several tumours.

The p16INK4A is an important G1/S cell cycle regulator gene [2]. RASSF 1A gene is involved in apoptotic signalling, microtubule stabilization and cell cycle progression [3]. The MGMT gene removes mutagenic and cytotoxic alkyl-adducts from the O6-position of guanine in DNA. Hypermethylation of the gene leads to the inactivation of DNA repair and to microsatellite instability [4].

To date, little is known about the exact role of hypermethylation of these genes in pancreatic adenocarcinoma, as the molecular mechanisms underlying these neoplasms remain poorly understood.

Article metrics: 519 views

Related Articles

Joydeep Ghosh, Meheli Chatterjee, Sandip Ganguly, Deepak Dabkara, Bivas Biswas
Luca Nicosia, Antuono Latronico, Mariagiorgia Farina, Anna Carla Bozzini, Paola Baratella, Viviana Enrica Galimberti, Stefano Fiori, Marta Montesano, Enrico Cassano
Maria Asunción Algarra, Maria José Juan Fita, Sergio Sandiego, Héctor Augusto Aguilar, Pablo Álvarez, Mateo Quispe, Antonio Salvador, Adoración Egido, Javier Lavernia, Isidro Machado, José Rubio Briones, Miguel Ángel Climent
Milagros Abad-Licham, Juan Astigueta, Caddie Laberiano Fernández, Himelda Chávez Torres, Grisnery Maquera Torres, Edwin Figueroa, Ricardo Bardales
Veronica Martini, Francesca D'Avanzo, Paola Maria Maggiora, Feba Maria Varughese, Antonio Sica, Alessandra Gennari
Elham Sajjadi, Konstantinos Venetis, Cristian Scatena, Nicola Fusco