A Cleveland Clinic-led research collaboration between Timothy Chan, MD, PhD, Chair of Cleveland Clinic’s Global Centre for Immunotherapy, and Bristol Myers Squibb has published the most comprehensive overview to date of how the immune system reshapes tumour architecture in response to immune checkpoint therapy.

The eight-year study, published in Nature Medicine, outlines how cancer immunotherapy induces tumour recognition through neoantigens to reshape the tumour ecosystem.

Neoantigens are small peptides produced when cancer cells mutate and are a primary marker for the immune system to recognise cancer cells as different from self.

“This study is unique in that we sampled tumours prior to therapy and then early after immunotherapy was initiated,” explains Dr. Chan, who is also chair of Cleveland Clinic’s Centre for Immunotherapy & Precision Immuno-Oncology, programme leader of Case Comprehensive Cancer Centre's Immune Oncology Programme and the Sheikha Fatima bint Mubarak Endowed Chair in Immunotherapy.

“Our goal was to understand how patients’ tumours are recognised and altered by their immune system in response to immunotherapy.”

The CheckMate-153 trial was overseen by pharmaceutical company Bristol Myers Squibb and Dr. Chan’s team was a central site for the trial’s analysis.

Within the primary trial, investigators included a biomarker sub-study to identify how neoantigens drive response to nivolumab by sampling patients’ tumours pre-therapy and 3 weeks post-therapy.

From these tumour samples, sequencing was used to identify mutations that create neoantigens.

Neoantigens are thought to be the primary way that the immune system recognises tumours, but neoantigen prediction tools lack accuracy due to lack existing data in this space.

To overcome this issue the team developed the largest neoantigen screen to date, where they validated their predictions and monitored the dynamic response to neoantigens with longitudinal blood draws.

Within three weeks of treatment, people who went on to respond well to nivolumab had a sharp decline in clonal neoantigens.

Meanwhile, individuals whose cancer did not go into remission still mounted an immunologic response but to smaller sub-clonal populations.

This is important because many believed that non-responders were unable to activate and recognise tumour, but here they show it may be that the immune system is mounting a response to neoantigens but that this is insufficient to destroy all tumour clones.

Current neoantigen prediction tools rely heavily on HLA-binding neoantigens, but they are missing the T cell recognition aspect of immunogenicity, says Cleveland Clinic’s co-first author Tyler Alban, PhD, Project Staff in the Chan Lab.

Dr. Alban, data scientist Prerana Parthasarathy, and others on the team developed a machine-learning programme that uses the new screening data to better predict immunogenic neoantigens.

In the process, the programme identified novel features harboured by these cancer-derived neoantigens. 

“We observed a whole ecosystem of immune cells at work, with each T cell recognising a different neoantigen altering the clonal makeup of the tumour,” Dr. Alban says.

“Our data let us generate new insights into neoantigens and resistance to immunotherapy.”

By cataloguing changes to neoantigens during treatment, Dr. Alban’s analyses challenged the prevailing theory in immunotherapy: that a tumour only needs one lucky mutation to develop features our immune systems recognise as a threat.

The results show that many different T cells recognising many different cancer-causing features are needed to respond well to treatment.

Roadmaps generated by these types of observational studies will be critical in navigating future immuno-oncology research, Dr. Chan says.

“Learning why our immune systems respond to some cancerous mutations but not others are like the holy grail for immunotherapy researchers,” he explains.

“Our findings are one of the closest things we have to figuring these things out.”

The group is also using their dataset in collaboration with IBM in the Cleveland Clinic - IBM Discovery Accelerator to more advanced AI models that predict new molecules for cancer treatments and cancer vaccine development.

Source: Cleveland Clinic