A new research paper was published in Oncotarget Volume 14 on September 15, 2023.
Patient-derived organoids (PDOs) and xenografts (PDXs) have been extensively studied for drug-screening.
However, their usage is limited due to lengthy establishment time, high engraftment failure rates and different tumour microenvironment from original tumours.
In this new study, researchers Dae Won Kim, Francisca Beato, Youngchul Kim, Alexandra F Tassielli, Ruifan Dai, Jason W Denbo, Pamela J Hodul, Mokenge P Malafa, and Jason B Fleming from Moffitt Cancer Center developed real time-live tissue sensitivity assay (RT-LTSA) using fresh tumour samples to overcome these limitations.
“To overcome the major hurdles of the PDX-based assay, we developed real time LTSA (RT-LTSA) using fresh tumour samples. In this study, we report a reliable and reproducible RT-LTSA with resected fresh tumour samples to predict patients’ clinical response to chemotherapy in pancreatic cancer.”
Tissue slices from resected pancreatic cancer samples were placed in 96-well plates, and the slices were treated with chemotherapeutic agents.
The correlation between the chemo-sensitivity of tissue slices and each patient’s clinical outcome was analysed.
The viability and tumour microenvironment of the tissue slices were well-preserved over 5 days.
The drug sensitivity assay results were available within 5 days after tissue collection.
While all 4 patients who received RT-LTSA sensitive adjuvant regimens did not develop recurrence, 7 of 8 patients who received resistant adjuvant regimens developed recurrence.
The researchers observed significantly improved disease-free survival in the patients who received RT-LTSA sensitive adjuvant regimens (median: not reached versus 10.6 months, P = 0.02) compared with the patient who received resistant regimens.
A significant negative correlation between RT-LTSA value and relapse-free survival was observed (Somer’s D: −0.58; P = 0.016).
“RT-LTSA which maintains the tumour microenvironment and architecture as found in patients may reflect clinical outcome and could be used as a personalised strategy for pancreatic adenocarcinoma. Further, studies are warranted to verify the findings.”
Source: Impact Journals LLC