Researchers have discovered that malignant rhabdoid tumours (MRT) - a rare paediatric cancer without effective treatments, may be sensitive to drugs that block the cancer cell’s ability to dispose of misfolded proteins.

The findings provide a much-needed therapeutic target for these and other cancers caused by mutations in the SMARCB1 gene.

Based on these findings, published today in Cancer Cell, the researchers now are leading a Phase II clinical trial to investigate this approach in renal medullary carcinoma (RMC), a related adolescent cancer also characterized by SMARCB1 mutations.

"Malignant rhabdoid tumours are aggressive paediatric cancers that generally manifest in children in the first year of life", explained Giannicola Genovese, M.D., assistant professor of Genitourinary Medical Oncology and corresponding author of the study.

"It’s somewhat fortunate that these are very rare cancers. With current treatments, the prognosis is ominous – just about one year,” said Genovese. “It’s an orphan disease, meaning it has no targeted treatments available. The current options include high-dose chemotherapy, radiation and surgery, which can cause significant developmental and neurological effects and increase the risk for secondary cancers later in life.”

Driven to answer the unmet needs for patients with MRT and other cancers caused by SMARCB1 mutations, the researchers developed a mouse model of MRT to study the disease biology and uncover vulnerabilities.

The researchers created a mosaic mouse model, meaning only a subset of cells carried the mutation, to study the loss of SMARCB1 in specific cells and tissues in the mice.

“Using an embryonic mosaic approach, we were able to overcome current limitations in technology and create a model that faithfully mimics features of human MRT,” said Alessandro Carugo, Ph.D., research scientist at the Institute for Applied Cancer Science (IACS). “We demonstrated, in our model, that SMARCB1-deficient cells are characterised by dysregulation of protein synthesis and disposal, causing them to be loaded with misfolded protein aggregates.”

Excess or misfolded proteins can be toxic to the cell, so the unfolded protein response (UPR) and endoplasmic reticulum (ER) stress response pathways are normally activated to clear protein waste.

However, with the loss of SMARCB1, these pathways became hijacked and were incorrectly activated to account for protein accumulation in malignant cells.

The researchers also discovered that the cancer cells were able to survive this buildup through the activity of p53, which is a tumour suppressor and inhibits tumour growth.

In contrast, SMARCB1-deficient cells appear to activate p53 for a survival advantage and, in fact, may benefit from chemotherapy treatments, which further stimulate p53 activity.

“TP53 is the most frequently mutated gene in cancer, but it does not appear to be mutated in MRT,” said Carugo. “We were excited to learn that, here, it’s functional because the cells need p53 to counterbalance the protein accumulation associated with SMARCB1 loss.”

Recognising the dysregulated protein pathways as a potential vulnerability, the researchers designed a drug combination to test this hypothesis.

Autophagy and proteasome degradation, which are both mechanisms for clearing protein waste were targeted.

The inhibition of these processes led to complete tumour regression.

These results were further confirmed in mouse transplants and cell lines derived from human MRT.

The findings of this study led the researchers to initiate a Phase II clinical trial testing a proteasome inhibitor in combination with chemotherapy for patients with RMC.

This rare form of kidney cancer, also driven by SMARCB1 mutations, affects adolescents and young adults - primarily African Americans with the sickle-cell trait.

The trial also is available for adolescents and adults who develop MRT in their kidney.

“This is the first trial designed specifically for SMARCB1-mutant RMC. These are rare cancers that don’t respond to the standard of care for other kidney cancers,” said Genovese. “There is a need for new therapies for treating these patients. I think we’ve developed a good platform for discovery, to provide options for these patients and hope for the families as well.”

The researchers are working to expand the trial in the future to include paediatric MRT and other SMARCB1-related cancers.

In addition, the team will continue to investigate new medicines that may benefit these patients in the future.

Source: University of Texas M. D. Anderson Cancer Center