Researchers have discovered an immune regulator that appears to dictate glioblastoma (GBM) progression by shutting down immune surveillance, indicating a potential new area of therapeutic investigation. 

Findings from the preclinical study led by Shulin Li, Ph.D., professor of Pediatrics, and Amy Heimberger, M.D., professor of Neurosurgery, were published in the online issue of Nature Communications.

Classical wisdom is that brain progression is linked to oncogene activation and suppressor gene inactivation; however genetic and epigenetic mutations are not the only cause of GBM progression,” said Li. “Some immune regulators can do the same thing and are key regulators of cancer, especially in certain tissues and environmental contexts

GBM, unlike melanoma and lung cancers, does not attract robust T cell immune responses, and, so far, immunotherapies have had little success against it. GBM is considered “immunologically cold” or unreactive likely due to tumour elaborated immune suppressive factors.

Findings from the study indicate FGL2 (fibrinogen-like protein 2), which is known for suppressing the immune system, is highly expressed in GBM. Investigators showed that inactivating or “knocking out” FGL2 from the tumour cells can eliminate tumour progression in mice with intact immune systems. Understanding this type of expression is key to discovering the causes of GBM progression.

First author Jun Yan, a research scientist, showed the FGL2 present in tumour cells controls a specialized group of dendritic cells which activates T cells. More specifically, FGL2 secreted from tumour cells prevents the differentiation of a special subpopulation of CD103 dendritic cells that are essential for triggering the activation of the tumour killing T cells. The study also showed these dendritic cells must find a way to the tumour microenvironment in the central nervous centre (CNS) in order to activate the T cells.

“This study is important because it shows that the immune system must interact in the CNS and in the tumour to be effective. Previously, this interaction was only thought to be necessary in specialised immune organs such as the lymph nodes,” said Heimberger. “It also shows a new mechanism of immune suppression that hasn’t been described before, and it further supports how important FGL2 is to this disease.”

The team also analyzed human GBM from The Cancer Genome Atlas and found that lower levels of FGL2 protein expression coupled with high levels of GM-CSF or IFN, DC differentiation inducer or T cell activator, are associated with longer survival of GBM patients.

Li and Heimberger are actively working on therapeutic strategies to target FGL2.

Source: MD Anderson Cancer Center