Our website uses cookies to improve your on-site experience. By using the website, cookies are being used as described in our Policy Document
Warning: To log in you will need to enable cookies and reload the page (Policy Document)
My ePortfolio Register   
 

Abstract | Full HTML Article | PDF ecancer 11 717 / https://doi.org/10.3332/ecancer.2017.717

Research

Circulating and tissue biomarkers in early-stage non-small cell lung cancer

Objective: We sought to characterise circulating and tissue tumour biomarkers of patients who developed early-stage non-small cell lung cancer (NSCLC) during long-term follow-up of a chemoprevention trial (NCT00321893).

Materials and Methods: Blood and sputum samples were collected from 202 high-risk asymptomatic individuals with CT-detected stable lung nodules. Real-time PCR was performed on plasma to quantify free circulating DNA. Baseline serum was investigated with a previously validated test based on 13 circulating miRNAs (miR-Test). Promoter methylation status of p16, RASSF1a and RARβ2 and telomerase activity were assessed in sputum samples. DNA was extracted from each tumour developed during follow-up and subjected to a mutation survey using the LungCarta panel on the Sequenom MassARRAY platform.

Results: During follow-up (9 years) six individuals underwent surgery for stage I NSCLC with a median time of disease onset of 20.5 months. MiR-Test scores were positive (range: 0.14–7.24) in four out of six baseline pre-disease onset sera. No association was identified between free circulating DNA or sputum biomarkers and disease onset. All tumours harboured at least one somatic mutation in well-known cancer genes, including KRAS (n = 4), BRAF (n = 1), and TP53 (n = 3).

Conclusion: Circulating miRNA tests may represent valuable tools to detect clinically-silent tumours. Early-stage lung adenocarcinomas harbour recurrent genetic events similar to those described in advanced-stage NSCLCs.

Keywords: non-small cell lung cancer; early detection; circulating biomarker; somatic mutation

Loading Article Metrics ... Please wait

Related articles

Review: The presence of secondary circulating prostate tumour cells determines the risk of biochemical relapse for patients with low- and intermediate-risk prostate cancer who are treated only with external radiotherapy

Abstract | Full Article | PDF | Spanish Published: 20 Jun 2018 / https://doi.org/10.3332/ecancer.2018.844

Research: The pattern of comorbidities in cancer patients in Lagos, South-Western Nigeria

Abstract | Full Article | PDF Published: 13 Jun 2018 / https://doi.org/10.3332/ecancer.2018.843

Research: Prostate-specific antigen-based screening in Afro-Caribbean men: a survey of members of the Caribbean Urological Association

Abstract | Full Article | PDF Published: 11 Jun 2018 / https://doi.org/10.3332/ecancer.2018.842

Case Report: Fatal interstitial lung disease caused by Panitumumab-containing chemotherapy regimen

Abstract | Full Article | PDF Published: 07 Jun 2018 / https://doi.org/10.3332/ecancer.2018.841

Review: Are aggressive epithelial cancers ‘a disease’ of Eutherian mammals?

Abstract | Full Article | PDF Published: 04 Jun 2018 / https://doi.org/10.3332/ecancer.2018.840



Founding partners

European Cancer Organisation European Institute of Oncology

Founding Charities

Foundazione Umberto Veronesi Fondazione IEO Swiss Bridge

Published by

Cancer Intelligence