Diagnosis and treatment decisions for a recently recognised type of children's brain tumour should be improved by the discovery of the genetic mechanism that causes it, say researchers who identified the unusual DNA abnormality in angiocentric gliomas.
Currently there is no definitive pathological test to help identify this rare type of low-grade glioma.
Named for their curious behaviour of "hugging" blood vessels in the brain, angiocentric gliomas are usually cured with surgery and don't need further treatment with radiation or chemotherapy.
But given their recent description and difficulties in identifying them confidently, some patients receive the additional therapy, which often is damaging to the growing brain, in an effort to prevent a recurrence.
"Now we know these angiocentric gliomas have a different biology, and we have an exact way of identifying them so that patients can avoid this additional therapy that has life-long consequences," said Rameen Beroukhim, MD, PhD, of Dana-Farber Cancer Institute, a senior author of the report in Nature Genetics along with Keith Ligon, MD, PhD, and Adam Resnick, PhD.
Ligon is a pathologist at Dana-Farber/Boston Children's Hospital Cancer and Blood Disorders Center and Dana-Farber/Brigham and Women's Cancer Center (DF/BWCC).
Resnick is an investigator at Children's Hospital of Philadelphia.
Angiocentric gliomas were recognised as distinct entities less than 10 years ago.
Their name reflects the tumour cells' tendency to line up around blood vessels.
Fewer than 30 cases have been described in medical literature.
Seizures are typically the first symptom leading to diagnosis.
Although they are classified as a tumour, they are not malignant and don't spread to other parts of the body.
These rare tumours fall under the umbrella of paediatric low-grade gliomas (PLGG), which collectively are the most common paediatric brain tumour.
Until now, no specific genetic abnormality had been identified as a "driver" of angiocentric gliomas.
In the current study, the researchers analysed data from 249 PLGG tumours, including 19 angiocentric gliomas, and discovered an unusual genetic accident as the fundamental cause of the angiocentric gliomas.
The culprit event is a shuffling of DNA segments that brings together two separate genes, MYB and QKI, which become joined, or fused. MYB is a "proto-oncogene" - a normal gene that can become a cancer-causing oncogene.
QKI is a tumour-suppressor gene that normally functions to prevent cells from becoming malignant.
When these two genes are abnormally joined, the researchers found, it triggers not one but three different mechanisms that converge to produce a tumour:
"This represents the first example of a single driver rearrangement simultaneously transforming cells via three genetic and epigenetic mechanisms in a cancer," the authors wrote.
They identified this specific abnormality as "a defining event" found only in the angiocentric gliomas - it was not present in any of the other paediatric low-grade gliomas examined in the study.
Because of these findings, the researchers said, angiocentric glioma should be classified as a separate biologic entity, with the presence of the gene fusion confirming the diagnosis.
"This could aid in distinguishing angiocentric glioma from tumours with higher potential for recurrence that could require further treatment," they said.
The authors have developed the first genetic test now available for these patients through collaboration with cytogeneticist Azra H. Ligon, PhD, of DF/BWCC.
Source: Dana-Farber Cancer Institute
(23 Jan 2017)
(23 Jan 2017)
(23 Jan 2017)