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Throwing molecular wrench into gene control machine leads to 'melting away' of leukaemia

Cancer researchers today announced they have developed a way of sidelining one of the most dangerous "bad actors" in leukaemia.

Their approach depends on throwing a molecular wrench into the gears of an important machine that sets genes into motion, enabling cancer cells to proliferate.

In tests in mice, the newly discovered method has resulted in what the researchers describe as the "melting away" of aggressive blood cancers while at the same time having no harmful impact on the function of normal cells.

The new research by Associate Professor Christopher Vakoc and colleagues at Cold Spring Harbor Laboratory (CSHL) is part of a broader effort in Vakoc's lab to fight the often fatal acute myeloid leukemia (AML) by disabling parts of the machinery in cells - called the transcriptional machinery -- that determines when genes are switched on and off.

Central players in this machinery are proteins called transcription factors, thousands of which are active in regulating genes across our chromosomes.

The question addressed in the new research, published today in Cancer Cell, was how to target one of the most troublesome transcription factors, called MYB.

It's an oncogenic, or cancer-inducing, transcription factor that enables cells to blow through the stop signs that normally prevent out-of-control growth.

"MYB is a dream target in cancer research," says Vakoc, "because it's involved in so many cancers; in leukaemia it's special because we know from previous research that by targeting MYB you can get AML not just to stop growing but actually to regress." Deactivating MYB in cancer has been a goal of many research labs..

Yali Xu, a Ph.D. student in the Vakoc lab leading the study, discovered how to selectively take MYB out of the picture in leukaemia by throwing a molecular wrench into the mechanism that the transcription factor normally activates.

First, the team discovered that MYB activates gene expression by docking at a giant gene-"co-activation" protein called TFIID (pronounced TF-two-D).

Next, they found a tiny weak spot on the massive protein.

This Achilles' heel, called TAF12, is a small, nub-like projection.

The team then tricked MYB into binding to short protein fragments, or peptides, that are shaped exactly like the place on TAF12 where MYB binds when it is promoting leukaemia.

A major achievement in the study was generating this peptide, which acts like a decoy.

Experiments in mice that model human AML showed that the peptide finds and binds MYB, preventing it from engaging the TFIID co-activator.

This resulted in mouse leukaemias shrinking in size by some 80% without causing harm to healthy cells.

While the peptide is not itself a drug, Vakoc says its action could be replicated by a drug. "It's a concept we're now discussing with the pharmaceutical industry. It is going to take lots of work before it can result in a medicine leukaemia patients might take. But we're excited about this new approach, because MYB is such an important player in many cancers and until now has eluded efforts to selectively target it."

Source: Cold Spring Harbor Laboratory

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