My ePortfolio Register   

Drug combination may boost immunotherapy responses in lung cancer patients

Johns Hopkins Kimmel Cancer Center researchers and colleagues have identified a novel drug combination therapy that could prime non-small cell lung cancers to respond better to immunotherapy.

These so-called epigenetic therapy drugs, used together, achieved robust anti-tumour responses in human cancer cell lines and mice.

During the study, published Nov. 30, 2017, in the journal Cell, a team of researchers led by graduate student Michael Topper; research associate Michelle Vaz, Ph.D.; and senior author Stephen B. Baylin, M.D., combined a demethylating drug called 5-azacytidine that chemically reignites some cancer suppressor genes’ ability to operate, with one of three histone deacetylase inhibitor drugs (HDACis).

The HDACis work against proteins called histone deacetylases that are involved in processes, such as cell copying and division, and can contribute to cancer development.

The combination therapy triggered a chemical cascade that increased the attraction of immune cells to fight tumours and diminished the work of the cancer gene MYC.

Based on these findings, investigators have launched a clinical trial of the combination therapy in patients with advanced, non-small cell lung cancer.

The development of therapeutic approaches for patients with lung cancer has been a critical medical need, says Baylin, the Virginia and Daniel K. Ludwig Professor of Cancer Research at the Kimmel Cancer Center.

While immune checkpoint therapy has been “a tremendous step forward, less than half of patients with lung cancer have benefited to date,” he says.

“In our study, the two-drug epigenetic therapy combination worked exceedingly well, even before putting in the immune checkpoint inhibitors,” Baylin says.

“In animal models of lung cancer, the two agents either prevented cancer from emerging or blunted the effects of more aggressive cancers. In both scenarios, a large component of the results involved an increase in immune recognition of the tumours.”         

In a series of experiments, researchers studied the combination of 5-azacytidine with the HDACis entinostat, mocetinostat or givinostat in human cancer cell lines and in mouse models of nonsmall cell lung cancers.

The treatments were found to alter the tumour microenvironment.

In cancer cell lines, 5-azacytidine worked against the cancer gene MYC, causing down regulation of the entire MYC signalling program.

Adding the HDACis further depleted MYC, and together the drugs subsequently caused actions that prevented cancer cell proliferation, simultaneously attracted more immune system T cells to the area of the tumour and activated these cells for tumour recognition.

In mouse models, the strongest response was observed when using 5-azacytidine plus givinostat.

In one mouse model with a mutant form of non-small cell lung cancer, this drug combination given for three months yielded prevention of benign, precursor tumours from becoming cancers and caused 60 percent reduction of overall area of benign tumour appearance in the lungs.

By contrast, a group of mice with the same form of lung cancer that were given a mock treatment universally developed large, cancerous lesions in the lungs.

In a second model of mice with established, aggressive, non-small cell lung cancer, treatment with an alternating schedule of 5-azacytidine with givinostat and of 5-azacytidine with mocetinostat not only reduced the growth of established, rapidly growing primary tumours but also dramatically reduced metastatic occurrence.

Source: The Sidney Kimmel Comprehensive Cancer Center

0

Comments

Please click on the 'New Comment' link to the left to add a new comment, or alternatively click any 'Add Comment' link next to any existing post to respond. The views expressed here are not those of ecancer. For more information please view our Privacy Policy.



Founding partners

European Cancer Organisation European Institute of Oncology

Founding Charities

Foundazione Umberto Veronesi Fondazione IEO Swiss Bridge

Published by

Cancer Intelligence