My ePortfolio Register   
 

Abstract | Full HTML Article | PDF ecancer 11 726 / DOI: 10.3332/ecancer.2017.726

Research

High-risk gastrointestinal stromal tumour (GIST) and synovial sarcoma display similar angiogenic profiles: a nude mice xenograft study

Background: Gastrointestinal stromal tumour (GIST) is the most common primary mesenchymal tumour of the gastrointestinal tract. Spindle cell monophasic synovial sarcoma (SS) can be morphologically similar. Angiogenesis is a major factor for tumour growth and metastasis. Our aim was to compare the angiogenic expression profiles of high-risk GIST and spindle cell monophasic SS by histological, immunohistochemical and molecular characterisation of the neovascularisation established between xenotransplanted tumours and the host during the initial phases of growth in nude mice.

Methods: The angiogenic profile of two xenotransplanted human soft-tissue tumours were evaluated in 15 passages in nude mice using tissue microarrays (TMA). Tumour pieces were also implanted subcutaneously on the backs of 14 athymic Balb-c nude mice. The animals were sacrificed at 24, 48, and 96 h; and 7, 14, 21, and 28 days after implantation to perform histological, immunohistochemical, and molecular studies (neovascularisation experiments).

Results: Morphological similarities were apparent in the early stages of neoplastic growth of these two soft-tissue tumours throughout the passages in nude mice and in the two neovascularisation experiments. Immunohistochemistry demonstrated overexpression of proangiogenic factors between 24 h and 96 h after xenotransplantation in both tumours. Additionally, neoplastic cells coexpressed chemokines (CXCL9, CXCL10, GRO, and CXCL12) and their receptors in both tumours. Molecular studies showed two expression profiles, revealing an early and a late phase in the angiogenic process.

Conclusion: This model could provide information on the early stages of the angiogenic process in monophasic spindle cell SS and high-risk GIST and offers an excellent way to study possible tumour response to antiangiogenic drugs.

Keywords: GIST, synovial sarcoma, angiogenesis, nude mice xenograft, chemokines

Categories:

Sarcomas

Loading Article Metrics ... Please wait

Related articles

Conference Report: Highlights from the 36th European Society of Surgical Oncology Congress (ESSO 36), 14–16 September 2016, Kracow, Poland: optimising European cancer surgery

Abstract | Full Article | PDF Published: 23 Mar 2017 / DOI: 10.3332/ecancer.2017.728

Research: VERO® radiotherapy for low burden cancer: 789 patients with 957 lesions

Abstract | Full Article | PDF Published: 29 Sep 2016 / DOI: 10.3332/ecancer.2016.677

Case Report: An extremely rare case of a high-grade pleomorphic cardiac sarcoma and likely cerebral metastasis in a young patient

Abstract | Full Article | PDF Published: 15 Aug 2016 / DOI: 10.3332/ecancer.2016.664

Case Report: Myxoid mesenchymal neoplasm presenting as massive arm and chest wall oedema with pleural effusion

Abstract | Full Article | PDF Published: 04 Nov 2015 / DOI: 10.3332/ecancer.2015.590

Conference Report: Conference report on the 28th annual meeting of the European Musculo-Skeletal Oncology Society, 29 April–1 May 2015, Athens

Abstract | Full Article | PDF Published: 08 Jul 2015 / DOI: 10.3332/ecancer.2015.550



Founding partners

European Cancer Organisation European Institute of Oncology

Founding Charities

Foundazione Umberto Veronesi Fondazione IEO Swiss Bridge

Published by

Cancer Intelligence